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KMID : 0984920140160020097
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Journal of Skin Barrier Research
2014 Volume.16 No. 2 p.97 ~ p.97
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Lipidomic Analysis of Skin Stratum Corneum using Direct-Infusion Nanoelectrospray Tandem Mass Spectrometry
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Liu Kwang-Hyeon
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Abstract
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Lipids are important components in all biological tissues. They play important roles associated with the proper function of the organism. Lipidomics, a branch of metabolomics, is a systems-based study of all lipids and their function within the cell. Shotgun lipidomics provide rapid semi-quantitative snapshots of the composition of complex lipidomes in samples. In this study, we developed lipidomics methodology for skin lipid profiling and identification using chip-based direct infusion nanoelectrospray tandem mass spectrometry. Skin ceramides are produced by coupling fatty acid acyl chains onto sphingoid bases by an amide binding. The MS/MS fragmentation pattern of skin ceramides was characterized through the interpretation of product ion scan mass spectra of them. Based on the MS fragmentation pattern of skin ceramides, an in silico MS/MS library for the annotation and identification of unknown lipids from skin samples has generated. This lipidomic platform was applied to identify the ceramide signatures in db/db mice as well as T2DM and allergic contact dermatitis patients. This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HN13C0076).
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KEYWORD
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